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Guaiazulene: biochemical activity and cytotoxic and genotoxic effects on rat neuron and N2a neuroblastom cells

Başak Toğar, Hasan Türkez, Ahmet Hacımüftüoğlu, Abdulgani Tatar, Fatime Geyikoğlu.

Aim: Neuroblastoma (NB) cells are often used in cancer researches such as glioblastoma cells since they have potential of high mitotic activity, nuclear pleomorphism and tumor necrosis. Guaiazulene (GYZ: 1,4-dimethyl-7-isopropylazulene) is present in several essential oils of medicinal and aromatic plants. Many studies have reported the cytotoxic effect of GYZ; however, there are no studies that compare such effects between cancer cell lines and normal human cells after treatment with GYZ. Method: In this study, we aimed to describe in vitro antiproliferative and/or cytotoxic properties (by 3-(4,5 dimetylthiazol -2-yl) - 2,5 diphenlytetrazolium bromide (MTT) test), oxidative effects (by total antioxidant capacity (TAC) and total oxidative stress (TOS) analysis) and genotoxic damage potentials (by single cell gel electrophoresis (SCGE)) of GYZ. Result: The results indicated that GYZ have anti proliferative activity suppressing the proliferation of neuron and N2a-NB cells at high doses. Additionally, GYZ treatments at higher doses led to decreases of TAC levels and increases of TOS levels in neuron and N2a-NB cells. On the other hand, the mean values of the total scores of cells showing DNA damage were not found different from the control values. Conclusion: From this study, it is observed that GYZ has in vitro cytotoxic activity against neuron and N2a-NB cells.

Key words: Guaiazulene, neuroblastoma, cytotoxicity, genotoxicity, MTT assay, SCGE assay

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