Abstract

ABSTRACT
The present study is to investigate the antimicrobial activity (antibacterial and antifungal) of protein hydrolysate of marine water molluscs Babylonia spirata (Linnaeus, 1758). Protein hydrolysate was prepared from tissue of Babylonia spirata by enzymatic hydrolysis. Enzyme digestion were carried out with the enzyme Trypsin. The protein concentration was estimated by Bradford’s method and the protein quantification was done by using SDS PAGE analysis. Antibacterial assay was carried out against four bacterial pathogens by agar well diffusion method and antifungal activity was performed against three human pathogenic fungal strains. 2.6mg/ml protein concentration was estimated by Bradford’s method and 40 to 200 kDa protein bands were resulted in SDS PAGE analysis.In antimicrobial activity, the maximum zone of inhibition was observed against Staphylococcus aureus 22.16 + 1.04 mm at 1000µg/ml concentration and the maximum zone of inhibition was observed in Aspergillus fumigatus 13.5+0.5 in 1000µg/ml concentration. These results are signify that the protein hydrolysate of marine molluscs Babylonia spirata express remarkable antimicrobial activity.

Key words: Gastropods, Babylonia spirata, Enzymatic digestion, Protein hydrolysate, Antimicrobial activity