A simple, highly sensitive stability indicating reverse phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of sumatriptan succinate in bulk and tablet dosage form. The analysis was performed on reverse phase C18 ODS Inertsil (250×4.6mm, 5µm) column, with a mobile phase containing buffer: aetonitrile: methanol (80:10:10 v/v/v), pH was adjusted to 2.5 with orthophosphoric acid (OPA) at 221nm, by an isocratic elution mode with 1ml/min flow rate using photo diode array (PDA) detector at ambient temperature. The injection volume and retention time was found 20Î¼l and 4.4minutes respectively. The method produced linear responses in the concentration range of 5-150 µg/ml, with a correlation coefficient of 0.999. The limit of detection (LOD) and limit of quantification (LOQ) values for HPLC method were found to be 1.967 and 5.961 Î¼g/ml respectively. The recovery of the method was 98% of the labelled value. This method was validated for accuracy, precision, linearity and robustness. Sumatriptan subjected to different ICH prescribed stress conditions of acid, alkali, peroxide, reduction, thermal, photolytic and humidity degradation. This method successfully applied to stability indicating method and also found to be routine quantitative analysis of sumatriptan by easily available materials with low cost.
Sumatriptan, reverse phase high-performance liquid chromatographic method, Stability indicating assay, method development and validation
Passive social media use and psychological well-being during the COVID-19 pandemic: The role of social comparison and emotion regulation.
Yue Z, Zhang R, Xiao J
Computers in human behavior. 2022; 127(): 107050
Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 209-220
Using the Health Belief Model to examine travelers' willingness to vaccinate and support for vaccination requirements prior to travel.
Suess C, Maddock J, Dogru T, Mody M, Lee S
Tourism management. 2022; 88(): 104405
Exploring the Alzheimer's disease neuroepigenome: recent advances and future trends.
Zhang H, Elefant F
Neural regeneration research. 2022; 17(2): 325-327
Environmental stocks, CEO health risk and COVID-19.
FernÃ¡ndez-MÃ©ndez C, Pathan S
Research in international business and finance. 2022; 59(): 101509