Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

AJVS. 2016; 49(1): 103-112

Application of Molecular Techniques to understand Pathogenesis of Equine Herpes Virus 9 (EHV-9) In Pregnant Hamsters

Osama M. Abas, Abdelkareem A. Mahmoud, Adel M. Khadr, Tharwat M. Elshemey, Amir H. Abdelrahman, Asmaa G .Saleh.

In the present study we tried to understand pathogenesis of equine herpes virus 9 (EHV-9) in pregnant animals through experimental infection of early and late pregnant Syrian hamsters which is considered the ideal experimental model for equidae. We described the clinical manifestations and tracked the viral load in different maternal tissues including blood, brain, uterus and placenta by using the absolute quantification technique of real time PCR. The viral load was expressed as the number of EHV-9 ORF30 gene copies and standardized against the house keeping hamster GAPDH gene. The viral load in the blood increased significantly at 6th day post infection regardless stage of infection, while it increased gradually in the brain tissue to reach maximum levels at late stages of pregnancy. In the other hand, the viral load in the uterine and placental tissues increased significantly in late trimester infected group than early trimester infected group. Clinical signs appeared on experimentally infected hamsters were very similar to that appeared in equines due to EHV-1 infection and it synchronically correlated to the viral load in different tissues. On the basis of our results we can conclude that clinical manifestations of EHV-9 are very similar to that of EHV-1 and its appearance is correlated to the increased viral multiplication in blood, brain, uterus and placental tissues of the infected animal.

Key words: EHV- 9, PCR, Viral load, EHV-1, Equine.

Similar Articles

Systematic review and meta-analysis of the papillomavirus prevalence in breast cancer fresh tissues.
Gomes de Oliveira G, Gonçalves AK, Eleutério J, Pinheiro LGP
Breast disease. 2022; 41(1): 123-132

Current trends in gene therapy for retinal diseases (Review).
Moraru AD, Costin D, Iorga RE, Munteanu M, Moraru RL, Branisteanu DC
Experimental and therapeutic medicine. 2022; 23(1): 26

Clinical Utility of Methylation-Specific Multiplex Ligation-Dependent Probe Amplification for the Diagnosis of Prader-Willi Syndrome and Angelman Syndrome.
Kim B, Park Y, Cho SI, Kim MJ, Chae JH, Kim JY, Seong MW, Park SS
Annals of laboratory medicine. 2022; 42(1): 79-88

Avian Paramyxoviruses as Vectors for Vaccine Development.
Kim SH, Shirvani E, Samal S
Methods in molecular biology (Clifton, N.J.). 2022; 2411(): 63-73

Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115(DE3) for Nontransgenic RNAi-Based Insect Pest Management.
Taracena ML, Garcia Caffaro I, Paiva-Silva GO, Oliveira PL, Rendon PA, Dotson EM, Pennington PM
Methods in molecular biology (Clifton, N.J.). 2022; 2360(): 279-294

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.

ScopeMed Web Sites