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Seasonal effects on water and osmotically-active ionic contents (Na+, K+ & Cl–) of skeletal muscles of the spiny-tailed lizard, Uromastix hardwickii

Muhammad Saleh Soomro, Muhammad Abdul Azeem, Muhammad Naeem Soomro, Rehana Rehman Rehman.

To assess the seasonal effects on water & osmotically-active ionic contents (Na+, K+ & Cl-) of skeletal muscles of the spiny-tailed lizard Uromastix hardwickii.
Fresh animals (adults) of both the sexes were used in all the experiments, and the gastrocnemius (skeletal) muscles were dissected out, reptilian buffer solution was used to keep dissected muscles moist & use during experimentation. The muscle sample solutions were prepared by heating/drying and incinerating/ashing the muscle tissues, treating with acid and deionized water respectively. These samples were then used for the estimation of Na+, K+ & Cl- ions. The water content was found as percent wet muscle weight.
The average mean values of water content and these osmotically active ionic contents were found to fall significantly(P

Key words: Colorimetric test (TPTZ method); Flame photometer; Osmolality parameters Na+, K+ & Cl-; Uromastix hardwickii; Water content

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