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Methanolic leaf extract of Gymnema sylvestre augments glucose uptake and ameliorates Insulin resistance by upregulating GLUT-4, PPAR-Ī³, adiponectin and leptin levels in vitro

Puttanarasaiah Mahesh Kumar, Marikunte V Venkataranganna, Kirangadur Manjunath, Gollapalle L Viswanatha, Godavarthi Ashok.

Aims: The present study was undertaken to evaluate the effect of methanolic leaf extract of Gymnema Sylvestre (MLGS) on Glucose transport and insulin resistance in in vitro.

Methods: PPARĪ³ and GLUT-4 expression was assessed in L6 myotubes for concluding the Glucose transport activity, and adiponectin and leptin expression was studied in 3T3 L1 murine adipocyte cell line to determine effect of MLGS (250 to 750 µg/ml) on insulin resistance.

Results: The findings of the experiments has demonstrated significant and dose dependent increase in glucose uptake in all the tested concentrations of MLGS, further the glucose uptake activity of MLGS (750 µg/ml) was at par with rosigilitazone (50 ļ­g/ml). Concomitantly, MLGS has shown enhanced GLUT-4 and PPARĪ³ gene expressions in L6 myotubes. Furthermore, cycloheximide (CHX) has completely abolished the glucose uptake activity of MLGS when co-incubated, which further confirmed that glucose uptake activity of MLGS was linked to enhanced expression of GLUT-4 and PPARĪ³. Additionally, in another experimental set MLGS showed enhanced expression of adiponectin and leptin, thus confirms the ameliorative effect of MLGS on insulin resistance.

Conclusion: These findings suggest that MLGS has an enhanced glucose uptake activity in L6 myotubes, and ameliorate the insulin resistance in 3T3 Ll murine adipocyte cell line in in vitro.

Key words: Gymnema sylvestre, Diabetes, Glucose transport, PPARĪ³, L6 myotubes, antidiabetic activity, Adiponectin , Leptin, 3T3 Ll murine adipocyte cell line.

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