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Ann Med Res. 1996; 3(4): 303-305

Screening of Blood Donors for HCV RNA by RT-PCR

Emine Sönmez, MD1, Catherine Troisi, PhD1, F. Blaine Hollinger, MD1, Hsiang Ju Lin, PhD1, Mohammed Naem, PhD1




A total of376 bood donors have been tested for anti-HCVby ELISA (second generation) test. 164 of 376 (40.95%) specimens were anti-HCV negative. 212 (59.05%) sera were anti-HCV positive. Seventy-six of total sera (20.21%) were found positive for HCV RNA. In 70 of 212 (33.01%) anti-HCV positive sera, HCV RNA was found positive. Six of164 anti-HCV negative sera (3.65%) were considered positive for HCV RNA. These results indicate that reactivity in second generation assays for hepatitis C virus antibodies correlates with viremia, but they are not sensitive enough to screen hepatitis C virus carriers with low viremia. [Journal of Turgut Özal Medical Center 1996;3(4):303-305]

KeyWords: HCV RNA, blood screening, HCV antibody

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