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Ann Med Res. 2002; 9(1): 29-31

Cultivation of Trichomonas intestinalis in CPLM media

Metin Atambay*, Ülkü Karaman*, Özlem M. Aycan*, Nilgün Daldal*




Trichomonas intestinalis is a flagellated protozoon which is located especially in the ileocecal part of the human

intestine and it is widespread throughout everywhere in the world. However it is said to cause rarely an

abdominal pain, mucoid diarrhoea periods and some deficiencies in the intestine, some searchers declared as it is


Diagnosis of T. intestinalis can be made by way of observing the motile trophozoids in the stool with direct

microscopic examination. And various kinds of methods are sugggested for cultivation of this protozoon.

In this study, the cultivation of T. intestinalis has been tried at CPLM media used widespreadly in the parasitology


T. intestinalis containing stool samples which were examined under light microscope in the Inönü University

Medical Faculty Department of Parasitology were cultivated in the CPLM media and reproduction was checked

every other day. It was observed that the T. intestinalis can survive for 16 days in CPLM culture.

As a result, CPLM medium is available for diagnosis of Intestinal Trichomaniasis and trophozoids may be used in

educational studies.

Key words: T.intestinalis, CPLM media

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