Introduction: The antioxidant capacity of CAPE (caffeic acid phenethyl ester) has been investigated in recent
years but, so far most of the studies were in vivo studies. In this study we investigated, the in vitro antioxidant
capacity of CAPE to scavenge superoxide radicals. CAPE, an active component of propolis from honeybee
hives, is known to have anti mitogenic, antioxidant, anticarcinogenic, antiinflammatory and immunomodulatory
Aim of the study: CAPE is an antioxidant molecule in vivo. The aim of this study is to investigate if CAPE under
in vitro conditions has the same effect similar to in vivo.
Methods: The method developed by McCord and Fridovich for the determination of SOD activity is modified by
replacing SOD with CAPE.
Results: We find that, CAPE has superoxide scavenging capacity in a dose dependent manner. The presence of
50 μmol/L CAPE caused 73.66 % percentage of inhibition in cyt c reduction.
Key Words: CAPE, Superoxide scavenging capacity, Oxidative stress.