Background: This study was designed to investigate the effects of duration and intensity of training on oxidant
status and antioxidant enzyme activities of muscle and erythrocyte.
Material and methods: Twenty five male Wistar-albino rats were divided into three groups as a control (n=10),
moderate (n=8) and strenuous (n=7) exercise. Moderate and strenuous exercise groups were trained for half an
hour and one hour throughout thirteen weeks, respectively. The gastrocnemius muscle tissues and the erythrocytes
were taken for spectrophotometeric analyses of SOD, CAT, GSHPx activities, and NO and thiobarbituric acidreactive
substances (TBARS) levels after the experimental procedure ended. Statistical evaluations were done by
Mann-Withney-U and Spearman rank tests.
Results: SOD and GSH-Px activities in erythrocyte of two experimental groups significantly increased, whereas
TBARS levels significantly decreased. Catalase activity only increased slightly in muscle tissue of moderate exercise
group. There was a negative correlation between SOD activity and NO level of gastrocnemius muscle in moderate
exercise group. In strenuous exercise group, we found negative correlations among SOD and GSHPx activities and
NO level in muscle and erythrocyte tissues.
Conclusion: Moderate exercise has great influence on antioxidant enzyme activities rather than the strenuous
exercise. However, strenuous exercise plays a regulator role in balance between oxidant and antioxidant status.
Key Words: Rat, Exercise, Erythrocyte, Gastronomies, Nitric oxide, TBARS, Antioxidant enzyme
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