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Original Research

Ann Med Res. 2004; 11(4): 207-211


The Effects of Methylene Blue on Lung Injury in Septic Rats

Semra Demirbilek*, E. Ebru Sızanlı*, Abdurahman Karaman**, Neşe Karadağ***, Nihayet Bayraktar****, Emine Türkmen***, M.Özcan Ersoy*

.




Abstract


 

Aim: The aim of this study is to investigate the effects of methylene blue (MB) on NO production, antioxidant

status and lipid peroxidation in lung injury during the different stages of sepsis in rats.

Material and methods:Sixty Sprague Dawley rats weighing 200-230 g, were randomly divided into 3 groups. The rats

of the first group were sham operated (control, group C); the second group, sepsis (group S); the third group

received MB (25mg/kg, i.p.), MBS (sepsis+MB). Sepsis was induced by caecal ligation and puncture method. Each

group was randomly subdivided into two subgroups (early and late sepsis) consisting of 10 rats which were

sacrificed at 9 or 18 h after surgical procedure. In the lung tissue, total nitrite/nitrate (NOX), superoxide dismutase

(SOD), catalyse (CAT), glutathion peroxidase (GSH-PX) and malondialdehyde (MDA) levels were measured.

Damage to the lung tissue was graded by a pathologist on a scale of 1 (best) to 4 (worst).

Results: In group MBS, CAT levels increased significantly both in early and late sepsis groups, whereas, SOD and

GSH-PX levels increased significantly only in early sepsis groups compared to group S. Methylene blue, significantly

decreased NOx and MDA levels in both early and late sepsis groups compared to group S (p






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