The Effect of Reperfusion Duration in Intestinal Ischemia-Reperfusion on Biochemical Parameters and Small Intestinal Anastomosis Healing
Gülsen Ekingen*, Canan Ceran*, Arzu Demirtola*, Billur Demiroğulları*, Banu Sancak**, Aylar Poyraz***, Kaan Sönmez*, A.Can Basaklar*, Nuri Kale*.
Aim: The aim of this study was to determine biochemical changes and evaluate anastomotic healing of small bowel in the early and late phase of reperfusion after intestinal ischemia.
Material and method: Thirty-six Wistar albino rats were divided into 6 groups. Control group (Laparatomy only), group I (40 minutes intestinal ischemia followed by 20 minutes reperfusion), group II (40 minutes intestinal ischemia followed by 24 hours reperfusion), control-A group (Intestinal anastomosis creation), group I-A (40 minutes intestinal ischemia followed by 20 minutes reperfusion and intestinal anastomosis creation), and group II-A (40 minutes intestinal ischemia followed by 24 hours reperfusion and intestinal anastomosis creation). Small bowel samples were obtained in control group, group I and group II at the end of the reperfusion period for tissue myeloperoxidase and superoxide dismutase levels. After creation of small bowel anastomosis in the fourth day postoperatively, bursting pressure and hydroxyproline content were determined in control-A group, group I-A and group II-A.
Results: Tissue myeloperoxidase and superoxide dismutase levels were increased significantly after 20 minutes reperfusion and than decreased to subnormal levels with in 24 hours of reperfusion (p