Objective: The aim of this study was to determine the predominant species, their distribution in clinical specimens and antibiotic susceptibility patterns of the commonly isolated agents in clinical specimens of patients in intensive care units between May 2005 and May 2006.
Material-method:One thousand nine hundred and fifty-nine specimens consisting of 1185 blood, 414 urine, 210 tracheal aspirates, 69 CSF, 29 catheter, 23 wound, 20 sputum and 9 sterile body fluid specimens were evaluated. Isolates were identified with miniApi systems after initial conventional tests. Antibiotic susceptibilities were determined by the disc diffusion method according to CLSI criteria.
Results: Five hundred and sixty-five pathogenic consisting of 276 Gram positive, 243 Gram negative bacteria and 46 Candida species were isolated. The most common isolates were coagulase negative staphylococcus (CNS) (194), Acinetobacter baumannii (56) Escherichia coli (53), Candida spp.(46) and Staphylococcus aureus (45), respectively. The methicilline resistance rates were 68.9 % for S.aureus and 64.4 % for CNS strains. Extended-spectrum ß-lactamase production rates were 24.5 % for E.coli and 60 % for Klebsiella pneumoniae. The most effective antibiotics were imipenem and aminoglycosides for Enterobactericeae and aminoglycoside antibiotics, especially netilmicin, for Pseudomonas aeruginosa and A. baumannii. Decreasing susceptibility rates for imipenem were determined among P.aeruginosa and A. baumannii strains.
Conclusion: These results emphasize the necessity for increasing the infection prevention measures and close follow-up of empirical antibiotic therapies in intensive care units.
Key Words: Intensive care unit, Nosocomial infection, Antibiotic susceptibility