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Original Research

Ann Med Res. 2008; 15(1): 5-7


Investigation of Demodex spp. By Histopathologic Method in Cases Involving Infectious and Benign States of the Skin

Ülkü Karaman*, Serpil Şener**, Tuncay Çelik*, Metin Atambay*, Engin Aydın***, Nilgün Daldal*

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Abstract


It has been reported that Demodex transmits through close contact between people and play a role in the pathogenesis of rosaceous, acne vulgaris, perioral dermatitis, seborrhoeic dermatitis, micropapular-pruritic dermatitis, and blepharitis.

Methods used for diagnostic purposes include cellophane tape, skin scraping, punch biopsy and standardized surface skin biopsy (SSSB). Mite density per cm2 is of importance to determine the pathogenesis of the parasite. 

This study aimed to investigate the detectability of Demodex spp. in histopathological examination of biopsies conducted through various preliminary diagnoses exluding neoplasma.  With this aim, 95 biopsy specimens which revealed no demodex in their histopathological examination were evaluated for positive demodex by getting stained with Hematoxylen-Eosin (HE) method, which  were positive in 41 (43.2 %) of the cases.

It was concluded that the preliminary stages of histopathological examination and staining procedure do not prevent the detection of demodex, but the density of the parasite should be measured with SSSB.

Key Words: Demodex spp, Histopathological diagnosis, Hematoxylen-Eosin






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