Home|Journals|Articles by Year Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Research

Stability indicating high-performance thin layer chromatography method for estimation of ascorbic acid in Hibiscus sabdariffa L. aqueous extract

Emanuel Lyimo Peter,Anita Kaligirwa,Tony lukwago.

Background: High performance thin layer chromatography (HPTLC) has become a routine analysis technique due to its advantages of low operating cost, high sample throughput and need minimum sample clean up.
Aim: To develop and validate an accurate, specific, repeatable and stability-indicating HPTLC method for the determination of ascorbic acid in presence of their degradation products for assessment of stability of the ascorbic acid in Hibiscus sabdariffa L. preparations.
Methods: Chromatographic sample separations employed thin layer chromatography plates (20 cm x10 cm) pre-coated with silica gel 60 F254 as a stationary phase. Solvent system consisted of acetone: toluene: water: glacial acetic acid (26:4:4:1 v/v) while scanning wavelength of 269 nm was used. Ascorbic acid was subjected to stress degradation conditions i.e. oxidation, dry heat treatment, photo degradation and hydrolysis under different PH. Method was validated according to international council for harmonisation guidelines.
Results: Peaks of products formed were well resolved with different Rf values. The linear regression data for the calibration plots showed good linear relationship with r2 = 0.9912 in the concentration range 2-4 g/ml. The mean value slope and intercept were -531.72 and 8040.22 respectively.
Conclusion: A rapid, simple, accurate and specific HPTLC method for determination of ascorbic acid in Hibiscus sabdariffa L. preparations was developed and validated. In addition, this method separated all the degradants from stress conditions showing that it is a stability indicating method. The developed method could now be used in quality control of H. sabdariffa herb products.

Key words: Ascorbic acid; Chromatography; Hibiscus sabdariffa; quality control

Full-text options

Full-text Article

Readers of this article also read the following articles
»Prevalence and Antibiotic Susceptibility of Methicillin Resistant Staphylococcus aureus in Colonized Health Care workers, Farm Workers and Dairy Cattle
»Towards Quantum Biology : The Ayurvedic Perspective
»Relation Between Global Environmental Issues And Surface Water Quality
»Evaluation of the use of whatsapp application for the workplace communication in the health sector by the AHP method
»Study to identify and rectify the causes of failure to administer Intra partum antibiotic prophylaxis in Group B streptococcus positive mothers and its effect on fetal outcome
»Histopathological spectrum of tumours of upper aero digestive tract in Surat - A study of 156 cases
»Results of Pulmonary Resection for Multi Drug Resistant Tuberculosis
»Submerged fermentation, purification and characterization of L-Asparaginase from Streptomyces sp. isolated from soil
»Waist-to-hip measurement ratio among dental students in urban areas
»Evans Syndrome
»Preoperative assesment and our surgical experience in patients with penile fracture

American Journal of Preventive Medicine and Public Health


BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.