Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

AJVS. 2016; 49(1): 55-59

Isolation and Molecular Identification of Egg Drop Syndrome 1976 (EDS-76) Virus in Egypt

Rawhia E. Hemida, Emad M. Al-Ebshahy, Samy A. Khalil.

To evaluate the current situation of infection with EDS-76virus in Egypt, a total of 35 field samples (15 kidneys, 16 oviduct and 4 oviduct swabs) were collected from 10 layer flocks raised in Menoufia, El-Behera and Gharbia governorates during the period 2014-2015. The flocks showed a decreased laying performance associated with the production of soft-shelled eggs. Virus isolation was attempted in 10-day-old embryonated duck eggs via allantoic sac inoculation.Five days after inoculation, the allantoic fluids were tested for the presence of hemagglutinating (HA) activity using chicken erythrocytes. Seven samples were tested positive for HA activity throughout three consecutive passages.The presence of EDS -76 virus was assessed in all HA-positive allantoic fluids by hexon gene-based conventional and real-time PCR assays. In 6 out of 7 samples, PCR was successfully applied to detect the virus DNA, meanwhile all the employed samples were tested positive for the presence of EDS-76 virus by real-time PCR assay. The SYBR green-based real-time PCR assay described in the present study was found to be more sensitive and less time consuming than conventional PCR assay for detection of EDS-76 virus genome.

Key words: Egg drop syndrome 76 virus, real-time PCR assay, laying hens, Egypt.

Similar Articles

Promoter CAG is more efficient than hepatocyte‑targeting TBG for transgene expression via rAAV8 in liver tissues.
Kang J, Hang L, Zheng W, Luo J, Zhang X, Song Y, Liu A
Molecular medicine reports. 2022; 25(1):

Growth differentiation factor 5: a neurotrophic factor with neuroprotective potential in Parkinson's disease.
Goulding SR, Anantha J, Collins LM, Sullivan AM, O'Keeffe GW
Neural regeneration research. 2022; 17(1): 38-44

New insights into Epstein‑Barr virus‑associated tumors: Exosomes (Review).
Chen W, Xie Y, Wang T, Wang L
Oncology reports. 2022; 47(1):

Safe Zones for Percutaneous Carpal Tunnel Release.
Wu PT, Chern TC, Wu TT, Shao CJ, Wu KC, Kuo LC, Jou IM
Hand clinics. 2022; 38(1): 83-90

Contributing factors common to COVID‑19 and gastrointestinal cancer.
Kostoff RN, Briggs MB, Kanduc D, Shores DR, Kovatsi L, Drakoulis N, Porter AL, Tsatsakis A, Spandidos DA
Oncology reports. 2022; 47(1):

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.

ScopeMed Web Sites