A sensitive, simple and economic spectrophotometric method was developed and validated for the determination of gabapentin in pure form and pharmaceutical preparations. The method is based on Schiff base condensation reaction of the primary amino group of gabapentin with salicylaldehyde reagent in presence of acetate solution at 45áµ’C for 20 mins. The obtained yellow colored derivative in methanolic medium showed absorption maxima at 403 nm. Under the optimum conditions, Beer’s law was obeyed in the concentration ranges of 6 to 100 Î¼g/mL with correlation coefficient value of 0.9961. Limit of detection and limit of quantification were found to be 1.16 and 3.48 Î¼g/mL, respectively. The validity of the described method was assessed according to the ICH guidelines. The mean percentage recoveries ± standard deviation were 100.57 ± 1.5 by applying the standard addition technique. The method was repeatable and precise (relative standard deviation â‰¤ 0.61% and â‰¤ 1.34%, respectively) and was successfully applied for determination of the investigated drug in its pharmaceutical dosage form without detectable interference from the additives, hence can be suggested for routine analysis of gabapentin.
UV-Vis, spectrophotometric, derivatization, gabapentin, salicylaldehyde.
Characterization and Analysis of Food-Sourced Carbohydrates.
Kiely LJ, Hickey RM
Methods in molecular biology (Clifton, N.J.). 2022; 2370(): 67-95
De Novo Sequencing of Heparin /Heparan Sulfate Oligosaccharides by Chemical Derivatization and LC-MS /MS.
Liang Q, Sharp JS
Methods in molecular biology (Clifton, N.J.). 2022; 2303(): 163-172
A ratiometric solid AIE sensor for detection of acetone vapor.
Zhang C, Zheng Y, Ding Y, Zheng X, Xiang Y, Tong A
Talanta. 2022; 236(): 122845
Derivatization of Soyasapogenol A through Microbial Transformation for Potential Anti-inflammatory Food Supplements.
Zhou X, Shen P, Wang W, Zhou J, Raj R, Du Z, Xu S, Wang W, Yu B, Zhang J
Journal of agricultural and food chemistry. 2021; ():
(-)-Epicatechin-An Important Contributor to the Antioxidant Activity of Japanese Knotweed Rhizome Bark Extract as Determined by Antioxidant Activity-Guided Fractionation.
Jug U, Naumoska K, Vovk I
Antioxidants (Basel, Switzerland). 2021; 10(1):