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Original Article



Antioxidant Aerva lanata Extract Supresses Proliferation and Induce Mitochondria Mediated Apoptosis in Human Hepatocelluar carcinoma cell line

Nevin Kottayath Govindan.




Abstract

Objectives: The aim of the present study was to investigate the anti-proliferative and apoptotic activity of petroleum ether extract of AervaLanata(ALpe) on Hep3B hepatocellular cancer cells. Materials and Methods: To evaluate the anti cancer potential of this plant,ALpe was prepared using petroleum ether (60-80) extraction. Total polyphenols, flavonoids and alkaloid concentration in ALpe was determined spectrophotometrically. Antioxidant activity of ALpe was tested against DPPH, ABTS and superoxide radicals in vitro. ALpewas tested for its cytotoxic potential against human liver carcinoma Hep3B cells using MTT assay. The effect of ALpeon the apoptotic inducing property was evaluated using AO/EB and DAPI staining. The ROS inducing ability of ALpewas determined using DCFH staining andMitochondrial membrane potential (MMP) was determined using Rhodamine 123 staining. Results: Experiments showed that ALpe have significant amount of biologically active components, polyphenols and alkaloids. ALpe showed stronganti proliferative activity and induced apoptosis of Hep3B cell lines as evident from AO/EB and DAPI staining. ALpe treatment increased the ROS production at higher concentration and reduced the MMP in Hep3B cells forcing to undergo apoptosis. Conclusion: The results indicate that ALpe with the presence of biologically active components can be used as apotential agent alone or in combination to treat liver related diseases.

Key words: Aerva lanata, Hep3B, Apoptosis, Proliferation.






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