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Egypt. J. Exp. Biol. (Bot.). 2015; 11(2): 181-188


OPTIMIZATION AND CHARACTERIZATION OF EXOPOLYSACCHARIDES FROM PLEUROTUS SALMONEO-STRAMINEUS AND ITS POSSIBLE APPLICATION

Eman H.F. Abd El-Zaher, Azza A. Mostafa, Soad M. Abu El-Souod, Enas M. El-Gaar.

Abstract
The present study revealed that the cultivation of Pleurotus salmoneo-stramineus on glucose yeast peptone medium at initial pH 6, sucrose and yeast peptone powder were used as sole carbon and nitrogen sources at 25C under submerged incubation enhanced EPS production. Also, data indicated that the addition of palmitic acid at concentration 0.2% was the best stimulator for EPS production, where 1.278 mg/ml was obtained of EPS dry weight from experiments. HPLC analysis explained that exopolysaccharides is composing of glucose, galactose, fructose, mannose, arabinose and xylose at concentration 186.26, 4.765, 0.398, 154.78, 0.105, and 0.178 mg/gm, respectively.1HNMR confirmed presence of α and β glycosidic linkages. EPS showed antioxidant activity 23.3% (25 mg/ml) and have antitumor activity against colon cancer cell DLD-1 where it inhibited tumour cell by 45%.

Key words: Exopolysaccharides, structure, production, biological activity, Pleurotus salmoneo-stramineus



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