Objective: To observe the effect of different extracts of Syzygium aromaticum (50% ethanolic and 50%
aqueous) on the weight of Streptozotocin (STZ) induced diabetic rats in comparison with insulin.
Study Design: Randomized control trial.
Place and Duration of Study: The study was conducted at National Institute of Health Islamabad from July 2011
to December 2011.
Materials and Methods: Forty adult rats of Sprague dawaley specie with weight ranging between 200-250 g
were selected and were equally divided into 5 groups (I-V) with eight rats in each group. Group- I was control.
Diabetes was induced to group II- V by a single intraperitoneal injection of STZ and rats with fasting blood
glucose above 200mg/dl were selected. After receiving the injection of STZ, the animals were weighed on Day
zero i.e 48 hours (post STZ ). Group-II was diabetic control , group III received 50% aqueous extract while
group IV received 50% ethanolic extract of Syzygium aromaticum at a dose of 750 mg/kg body weight
respectively for sixty days. Group V received 70/30 humulin insulin, 0.6 units/kg body weight subcutaneously,
bid for sixty days. After two months of study on Day 60 the animals were weighed again, changes in the weight
which occurred with both extracts were compared with the changes in the weight which occurred with the
standard drug insulin.
Results: Group-IV receiving 750 mg/kg body of 50% ethanolic extract showed marked improvement in the
weight as compared to group III receiving 50% of the same dose of aqueous extract. Group V receiving insulin
showed improvement in the weight which is almost closer to the increase in weight which occurred in group III
Conclusion: 750 mg/kg body weight of 50% ethanolic extract of Syzygium aromaticum caused more
improvement in the weight of diabetic rats than the increase in the weight with the 50% aqueous extract of the
same dose and the drug insulin.
Diabetes Mellitus, Weight Improvement , Syzygium Aromaticum Extract.