In vitro anti-oxidant and in vivo anti-inflammatory activity determination of the methanolic leaves extract of Millettia pachycarpa
Shofiul Azam, Prawej Ansari, Mohammad Mamun Ur Rashid, Mohammad Nazmul Alam, Ismail Hussein Ahmed, Mubarik Yusuf Ibarahim, Shah Mohammed Shafi, Sadequr Rahman, Altaf Hossen.
Objective: Millettia pachycarpa is a member of the Fabacea family and our object has been set to examine the anti-oxidant and anti-inflammatory endeavor of this plant. This plant has been reported as potential anti-inflammatory agent previously.
Materials and Method: DPPH free radical scavenging and reducing power assay was carried out to assess the electron donating capability of the methanolic crude extract of the specimen. A quantitative analysis has been made to measure phenolic and flavonoid content. The anti-inflammatory potentiality of methanolic extract of M. pachycarpa has been determined via carrageenan-induced paw edema assay in rats.
Results: The IC50 value estimated was, 196.47 ± 2.061 µg/ml, which is a very much promising result. From quantitative assessment, sample extract reported very good number of phenolic and flavonoid measurement, 41.23 ± 1.527 mg equivalent gallic acid per gram and 110.33 ± 2.255 mg equivalent rutin per gram, respectively. The studied specimen showed expected dose dependent result in anti-inflammatory assay. At the dose of 300 mg/kg the extract showed maximum inhibitory effect (61.36% at 2 hour; P
Key words: anti-inflammatory, anti-oxidant, Carrageenan, DPPH scavenging, flavonoid, M. pachycarpa