ABSTRACT
Oxidative stress (OS) leads to deleterious effects in organisms. Main cause for the oxidative stress is the generation of free radicals in the cells. Antioxidants have ability to scavenge and neutralize the free radicals. Therefore, present study has undertaken to test the antioxidant potential of Phyllanthus amarus ethanolic extract (PAE) in Drosophila melanogaster through biochemical analysis. OS test has been conducted in the PAE supplemented different age grouped flies by exposing to 20 mM acrylamide (ACR). Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) were measured in different age grouped stressed and non-stressed PAE supplemented flies. OS test results revealed that PAE II treated groups showed significantly increased ACR resistance when compared to PAE I and control groups. All the age grouped PAE supplemented flies in non-stress conditions had significantly high SOD and CAT activities. Highest SOD and CAT activities were observed in PAE II treated 30 and 45 days flies respectively. Under stress conditions, the elevated levels of SOD activities were found in 30 and 45 days and CAT activities in 30, 45 and 60 days aged PAE treated flies. Overall data highlights that there is a dose dependant relationship between the extract concentrations and enzyme activities.
Key words: Keywords: Phyllanthus amarus, Drosophila melanogaster, Oxidative stress, Acrylamide, Antioxidant enzymes
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