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Original Article

J Liaquat Uni Med Health Sci. 2013; 12(2): 87-90

Two Easy and Early Methods for the Diagnosis of H. Pylori i.e: Serum Anti H. Pylori Antibodies and Stool Antigen

Rasheed Ahmed Soomro, Tanveer Ahmed Sheikh, Amir Iqbal Memon, Nazir Ahmed Pathan, Nasreen Qazi.

OBJECT: Two easy methods for the diagnosis of h pylori i.e: Serum Anti H Pylori Antibodies & Stool Antigen.
MATERIALS AND METHODS: Hundred serum and stool samples collected from the department
of surgery, and Medicine were registered at Department of Pathology and Diagnostic and Research Lab LUMHS, Jamshoro/ Hyderabad for performing H. pylori diagnosis were included in
this Study. Patients of all age groups and both sexes with complain of dyspepsia, or having the
clinical suspicion of H. pylori associated gastritis were included. Patients having H/O NSAIDs,
or eradication therapy were excluded. A specially designed proforma was used for recording
the relevant data. The Period of study was 15-10-2009 to 28-02-2011.
RESULTS: From 100 cases of, H. pylori antibodies were found in 68(68 %). 38(55.9%) were male
and 30(44.1%) were females. Sensitivity of this technique was 75.55% and specificity 60%. p
value 0.007. From stool samples collected from 100 patients H. pylori antigen were positive in
48 (48%) cases detected on immunochromatography technique. Stool antigen positive was in 28
(58.3%) males and 20(41.7%) Female patients. The stool antigen sensitivity was 52.74% and
specificity 90% P value (0.011).
CONCLUSION: H. pylori positivity is highly significant in histological examination of endoscopic biopsies on H&E and Giemsa Stains The detection of anti H. pylori antibodies from
serum by ELISA method and the H. pylori antigen from stool samples by immunochromatographies method, can be non invasive simplest, least expensive and alternatives in patients
unwilling for endoscopy.

Key words: Helicobacter pylori (H. pylori), endoscopy, serological and stool antigen HpSA, and Anti H. pylori IgG antibodies, MALT lymphoma (mucosa associated lymphoid tissue lymphoma).

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