Polymorphisms in CCL2 and IL6 genes in men with unstable atherosclerotic plaques in the coronary arteriesEvgeniia Striukova ,Vladimir Maksimov,Yulia Ragino,Yana Polonskaya,Ivan Murashov,Aleksandr Volkov,Aleksej Kurguzov,Aleksandr Chernjavskii,Elena Kashtanova.
The goal of research. To study polymorphisms of the CCL2, IL6 genes in order to find their associations with the presence of unstable atherosclerotic plaques in the coronary arteries (CA) and with protein levels of MCP-1, IL-6, factor XII, endothelin-1 in blood.
Materials and methods. The study included 101 men aged 40-70 with coronary atherosclerosis. According to histological analysis of plaques (obtained by endarterectomy from CA), all men were divided into 2 groups: 40 men (39.6%) with stable atherosclerotic plaques, 61 men (60.4%) had unstable plaques in the CA. Biochemical studies were performed by enzyme immunoassay. Genotyping was performed by PCR with RFLP.
Results. Single nucleotide polymorphisms rs1024611 of the CCL2 gene, rs1800795 of the IL6 gene are not associated with the presence of unstable atherosclerotic plaques in coronary arteries. Levels of biochemical inflammatory markers MCP-1, IL-6 do not differ in groups with different genotypes of CCL2, IL6 genes. Differences were found in the groups of patients with stable and unstable plaques in factor XII and endothelin-1 levels in patients with different CCL2 and IL6 genotypes.
Conclusion Single nucleotide polymorphisms rs1024611 of the CCL2 gene, rs1800795 of the IL6 gene are not associated with the presence of unstable atherosclerotic plaques in coronary arteries.
Key words: rs1024611, rs1800795, endothelial dysfunction factors, stable and vulnerable atherosclerotic plaques in the coronary arteries