Abstract

The main objective of this research was to determine the effect of consumption of corn sprouts and soybean sprouts formulas enriched with eggshells as a source of calcium corn and soybean sprouts with eggshells (CSSEs), on the improvement of oxidative stress and estrogen hormone depletion in ovariectomy (OVX) rats. The animals used Sprague Dawley rats were divided into four groups, (i) without OVX (NC); (ii) OVX control (OVX-C); (iii) OVX+ethynylestradiol (OVX-E); (iv) OVX+CSSE (OVX-K). All treatments were given orally for 6 weeks. After 6 weeks, the OVX group experienced a higher increase in body weight than the NC group. The results of serum analysis showed that the control group (NC) had the highest estradiol level and the lowest in OVX-C, while the OVX-E and OVX-K groups showed an increase in serum levels of estradiol after treatment. The addition of estradiol and CSSE increased serum Ca and P. After the treatment, there was an increase in superoxidase dismutase (SOD) levels in the OVX-E and OVX-K groups, and vice versa in the OVX-C group decreased. Serum malondialdehyde (MDA) levels decreased after treatment in the OVX-E and OVX-K groups and increased in the OVX-C groups. CSSE consumption improved estrogen depletion and oxidative stress with SOD and MDA markers and increased Ca and P in postmenopausal ovariectomized rats.

Key words: Corn and soybean sprouts; oxidative stress; estrogen depletion; ovariectomized