Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Article

J App Pharm Sci. 2015; 5(12): 074-079

Assessment of Chemical Disinfectants Efficacy against Escherichia coli Biofilm Developed on Glass and Wood at Refrigeration and Room Temperatures

Anas Muazu, Nor Iza A. Rahman, Umar Faruk Abdullahi, Sani Aliyu, Jonathan Ajisafe Ogidi, Ahmed Faruk Umar.

Objectives: The aim of this work was to study the formation of biofilm on glass and wood coupons at refrigeration and room temperatures, different incubation periods and to assess the efficacy of hydrogen peroxide (HP), Para Acetic Acid (PAA), Sodium hypochlorite (SH) and mixture of PAA + SH against the biofilm. Method: 200 L of 108 suspension E. coli ATCC 29922 was inoculated on the coupons inside petri dishes containing 20 ml of tryptic soy broth, incubated at 10 and 270 C for 24, 48, 72 and 168 hours. Biofilm developed at each hour above was quantified by bead-vortex followed by agar plating. The action of disinfectants was tested on 168 hours biofilm. The surfaces were exposed to the disinfectants and incubated at 270 C for 10 minutes, followed by deactivation for 5 minutes. Cells that resisted disinfectants effect were vortexed and enumerated by agar plating. Results: The results showed that E. coli can form high biofilm on wood in addition to glass. After disinfection treatment, HP had the highest efficacy at 270 C followed by PAA then SH, whilst PAA + SH had the least. Conclusion: it can be concluded that HP and PAA can be good disinfectants agents against E. coli biofilm.

Key words: Biofilm, E. coli, Log Reduction, Survival Fractions

Share this Article

Archives of Clinical and Experimental Surgery (ACES)


BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.