Diabetes, is a long-term metabolic illness typified by high blood sugar levels as a result of insulin-related issues. This study aimed to evaluate the In-vitro and in-vivo antidiabetic activity of Annona muricata L (Sour soup) methanol leave extract (AMMLE) in albino rats. In vitro studies were carried out using α-amylase and glycosylated hemoglobin inhibition assay. LD50 and phytochemical screening of AMMLE were determined using the standard methods. The sub-chronic effect of AMMLE on body weight, antidiabetic, and antioxidant of the pancreas was evaluated in alloxan-induced diabetic rats The results of the inhibition of glycosylation of hemoglobin of AMMLE showed a lower IC50 value (10.00, 7.10, 4.58 mg/ml) when compared with the positive control, Metformin (11.40,10.00 and 7.67mg/ml). α-amylase inhibition assay revealed that the inhibitory activity (IC50) of AMMLE (0.40mg/ml) was higher when compared with the positive control (VogliboseIC50 value of 0.26mg/ml). The acute toxicity study of AMMLE did not show mortality in the animals at the limit dose of 3000mg/kg during the observed period. The phytochemicalrevealed numerous bioactive components(Alkaloids, Saponins, Steroids, Tannins and Cardiac glycoside). Alloxan were capable of inducing diabetic status as there was a significant increase (P˃0.05) in all the treated groups compared to normal control. The AMMLE and the standard drug showed a significant (P˃0.05) decrease effect on fasting blood glucose in alloxan-induced rats, compared to diabetes control groups. There is a significant (p
Key words: Annona muricata L., Methanol leaf extract, Albino rats, Antidiabetic activity, In vitro study, In vivo study
|
