New Delhi metallo-β-lactamase (NDM-1), as a broad-spectrum β-lactamase, is able to inactivate almost all β-lactams. NDM-producers have been detected in extensively pathogen worldwide. The objective of this study was to analyze the homology of NDM-1-encoding genes based on the blaNDM-1 gene sequences downloaded from GenBank. The phylogenetic tree analysis showed that the blaNDM-1 gene sequences divided into two different branches with similarity score only 54. A common sequence of 242bps at length was extracted from branch-1, and 469bps common sequence was extracted from branch-2, respectively. The subsequent detection of NDM-1 harboring clinical isolates with PCR and real-time PCR molecular techniques should based on the two common sequences of branch-1 and branch-2.
Key words: New Delhi metallo-β-lactamase (NDM-1), Emerging pathogen, Resistance, Homology analysis, sequences contrasting
|
Download PDF 
Post
