The present study was carried out with six wheat and four maize genotypes from Mahyco Research Centre, Dawalwadi, Jalna, MH to develop wheat double haploid lines by wheat x maize crossing system. The following steps were followed while conducting the experiment: emasculation of wheat spikes, crossing or pollination of emasculated spikes with maize pollens, treating pollinated spikes with 100 ppm 2,4-D solution, excision of embryo and subsequent culture on half strength MS media. In total, 296 spikes were emasculated and pollinated with randomly collected maize pollens after 24 hrs. Success was obtained in rescuing a total of 27 embryos from 575 caryopses among two genotypes i.e. MW2 and MW3. Percentage of embryo formation varied in the range of 0-10 per cent owing to non-standard conditions. However, the frequency of embryo formation could be increased by focusing on the most important of all culture conditions i.e. temperature (18-21 C).
Key words: Wheat, maize, haploid, wheat x maize cross system, 2, 4-D
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