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Original Article

J App Pharm Sci. 2015; 5(Supplement 3): 084-090

Simple Method for Measuring Endothelial Nitric Oxide Synthase Activity in Clinical Researches

Khaled sobhy abdelkawy, khaled abdelaziz Salem.

This study aimed to evaluate a new simple colorimetric method for the assay of endothelial nitric oxide synthase activity in clinical researches and to compare between vanadium chloride and nitrate reductase enzyme in the assay method.

Methods and materials:
The method included using RBCs lysate to measure eNOS of the endothelium, using NADPH recycling system to enable the colorimetric measurement of eNOS, and using vanadium chloride to converting nitrate to nitrite. Vanadium results were compared with nitrate reductase results. The clinical study involved sixty patients who were randomly divided into two groups; group one received atorvastatin 40 mg daily, group two (control group) received placebo capsules. Patients were examined both before and after six weeks for endothelial nitric oxide synthase (eNOS) and for international index of erectile function-5 (IIEF-5) scores.

Results: In comparison to nitrate reductase, vanadium showed no significant difference in eNOS activity with similar activity. However, In comparison to control group, atorvastatin showed a significant increase in eNOS, (48.16% for vanadium, and 44.3% for nitrate reductase, P

Key words: endothelial, Nitric oxide synthase, vanadium, nitrate reductase, red blood cells

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American Journal of Physiology, Biochemistry and Pharmacology


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