Abstract
Introduction: Environmental pollution is a worldwide problem in a modern society. The extensive uses of pesticides are widely used to enhance the crop production and other benefits and have raised concerns about potential adverse effects on the environment, human health and non-target animals. Imidacloprid is one of the major representatives of the new generation of neonicotinoid insecticides derived from nicotine isolated from the tobacco plant. Imidacloprid is a widely applied pesticide due to their higher affinity for insect nicotinic acetylcholine receptors, it acts on nervous system. Worldwide, it is considered to be one of the insecticides used in the largest volume.
Methods: Present study was carried out in the department of Anatomy Govt. Medical College, Ambedkar Nagar and Santosh Medical College Ghaziabad U.P. on 270 fertile eggs of white leghorn chicken obtained from government poultry farm after taking permission from animal ethical committee. Chicken eggs exposed to Imidacloprid with doses of 12.5µg, 25µg, and 50µg in a volume of 12.5µl, 25µl and 50µl respectively and control same as test group. The embryos were terminated on 21st day, egg shell broken with a scalpel and embryos removed. Biochemical and developmental changes observed and recorded.
Results: The results show that experimental into failure of retraction of yolk sac, limbs defects, and Ectopia Viscerale and biochemical changes as compared to controls.
Conclusion: Imidacloprid exposure increases the risks of developmental defects and biochemical parameters alterations with increasing embryonic age. Comparatively higher doses proved more toxic and also caused many developmental defects in chick embryos.

Key words: Imidacloprid, chick embryos, developmental and biochemical effects.

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