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Original Article



Ex-vivo cytotoxic, antibacterial and DPPH free radical scavenging assay with ethanolic leaf extract of Glycosmis pentaphylla to justify its traditional use

Prawej Ansari1,2*, AKM Riasat Ul Islam2, Anaytulla1, Mahmuda Sultana3, Mohammad Nazmul Alam1, Mohammad Mustakim1, Md. Nasir Uddin4.

Abstract
Aim: Glycosmis pentaphylla belongs to the family Rutaceae. It is a shrub and locally common in the treatment of hepatic impairment. We have designed this study to provide a scientific basis with the traditional use of leaf of G. pentaphylla in the treatment of hepatitis.Methods: The well-established DPPH free radical scavenging activity was tested for antioxidant property evaluation. On the other hand, disk diffusion and brine shrimp method was respecti-velyused to determine antibacterial and cytotoxic activity. Results & Discussion: In the evaluation of antioxidant property IC50 found 204.91 2.223g/ml, in cytotoxicity testing, it is found that the plant part shows 30.49 1.976 g/ml of LC50. The ethanolic extract of G. pentaphylla leaves also have efficiency in bacterial growth inhibition; this extract is effective against for both gram, negative and positive. The zone of inhibition at 500 g/ml dose in E. coli and C. albican culture was 18 mm and 15 mm, respectively. In thin layer chromatography analysis, we found presence of couple of non-polar and polar component, presence of three non-chromatophoric component are also evident.Conclusion: Appropriate isolation and identification of mechanism is suggested in further study.

Key words: Antimicrobial; Antioxidant; Cytotoxic; G. pentaphylla; TLC.



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