Ex-vivo cytotoxic, antibacterial and DPPH free radical scavenging assay with ethanolic leaf extract of Glycosmis pentaphylla to justify its traditional usePrawej Ansari1,2*, AKM Riasat Ul Islam2, Anaytulla1, Mahmuda Sultana3, Mohammad Nazmul Alam1, Mohammad Mustakim1, Md. Nasir Uddin4.
Aim: Glycosmis pentaphylla belongs to the family Rutaceae. It is a shrub and locally common in the treatment of hepatic impairment. We have designed this study to provide a scientific basis with the traditional use of leaf of G. pentaphylla in the treatment of hepatitis.Methods: The well-established DPPH free radical scavenging activity was tested for antioxidant property evaluation. On the other hand, disk diffusion and brine shrimp method was respecti-velyused to determine antibacterial and cytotoxic activity. Results & Discussion: In the evaluation of antioxidant property IC50 found 204.91± 2.223µg/ml, in cytotoxicity testing, it is found that the plant part shows 30.49 ± 1.976 µg/ml of LC50. The ethanolic extract of G. pentaphylla leaves also have efficiency in bacterial growth inhibition; this extract is effective against for both gram, negative and positive. The zone of inhibition at 500 µg/ml dose in E. coli and C. albican culture was 18 mm and 15 mm, respectively. In thin layer chromatography analysis, we found presence of couple of non-polar and polar component, presence of three non-chromatophoric component are also evident.Conclusion: Appropriate isolation and identification of mechanism is suggested in further study.
Key words: Antimicrobial; Antioxidant; Cytotoxic; G. pentaphylla; TLC.