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Original Article

J App Pharm Sci. 2015; 5(10): 113-117


Isolation and Characterization of a Lactose-Binding Lectin from Ocimum sanctum

Praveen Kumar Vemuri, Bhavana Talluri, Ananya Sharma, Geethika Akkala, Vijaya Lakshmi Bodiga.




Abstract

A novel lectin has been isolated from Ocimum Sanctum and purified to homogeneity by gel filtration chromatography. The protein eluted as a single symmetrical peak from a Biogel P-100 column with an apparent molecular mass of 66 kDa. The lectin had a specific agglutinating activity with human erythrocytes at a minimum concentration of 0.7µg/ml. The lectin was stable in the pH range 5-12 and at temperature less than or equal to 800C for 30 min. The lectin isolated from Ocimum Sanctum had shown highest agglutinating activity at pH 7 and 250C after one hour incubation. Of the various sugars tested, even at 1000 mM sugar concentration, no inhibition was observed. The isolated lectin was found to be a lactose-biding lectin and found to contain 2.6 mg/ml of total sugar and 9.3 mg/ml of total protein

Key words: Lectin, Blood group, Glycobiology, ELISA, Agglutination, Ocimum sanctum






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