Home|Journals|Articles by Year Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Article

J App Pharm Sci. 2015; 5(Supplement 3): 022-026

Phytochemical screening and evaluation of biological activity of Calligonum polygonoides L. subsp. comosum

Hayam Ahmed, Abeer Moawad, Asmaa Owis, Sameh Abouzid, Rehab Abdel-Rahman.

Calligonum polygonoides L. subsp. comosum; locally known as “arta”, is tall woody shrub, perennial desert plant. TLC screening, estrogenic and antimicrobial activity of different fractions of leaves alcohol extract; n-hexane, CH2Cl2, EtOAc and n-BuOH has been studie in order to find the correlation between the phytoconstituents and the biological activity. Estrogenic activity was studied in immature ovariectomized female Wistar rats by oral administration of 75 and 150 mg extract/kg body weight for seven days using 1 µg estradiol/rat/day as positive control. The antimicrobial activity against Aspergillus fumigatus, Candida albicans, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Klebsiella pneumonia was done using agar diffusion method with determination of the minimum inhibitory concentration (MIC). CH2Cl2 fraction showed significant estrogenic and antimicrobial activity by significantly increasing uterine weight and low MIC values for all tested microorganisms ranging from 0.03 and 3.9 mg/mL. The n-hexane fraction showed mainly the presence of sterols and/or triterpenoids. EtOAc and n-BuOH were the richest in flavonoids while that of methylene chloride was found to contain both classes of chemical compounds. In conclusion; flavonoids, sterols and/or triterpenes exhibit synergistic effect to the antimicrobial and estrogenic activity of Calligonum polygonoides leaves.

Key words: Calligonum polygonoides, TLC screening, estrogenic, antimicrobial

Full-text options

Full-text Article

Archives of Clinical and Experimental Surgery (ACES)


BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.