Soybean is an essential legume crop grown worldwide due to its high nutrient value and higher percentage of protein contain. Various pathogens and pests cause diseases which result in low yield of soybean crop. Bacterial blight and soybean mosaic are the two most important diseases of Glycine max which affect the crop production. Plants protect themselves from these attacks through various mechanisms, one of which involves R (resistance) gene which provides resistance to plant against various diseases for instance; Rpg1b gene (type of nucleotide-binding sites-leucine-rich repeats [NBS-LRR] genes) provides resistance against bacterial blight disease in soybean. R genes cloning is the most recent method for obtaining disease resistance legume varieties. The genome of William 82 cultivar of G. max is completely sequenced but very little is known about the genome of Indian soybean varieties. Thus, identification of Rpg1b gene analogs in Indian variety is beneficial in breeding programs of soybean. Genomic DNA was extracted from selected genotypes various Indian soybean cultivars using CTAB method, purified and amplified using primers designed for NBS and LRR regions of Rpg1b genes. Both primers showed amplification in selected cultivars which confirmed the presence of Rpg1b gene in them. After gene sequencing, comparison of these sequences was done with Williams 82 cultivar using box shade tool which showed variations among them. This study on Rpg1b gene in various Indian soybean cultivars will be beneficial for the development of R gene-based molecular markers linked to bacterial blight in soybean.
Key words: Glycine max, Rpg1b, disease resistance genes, NBS-LRR, Bacterial blight
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