Abstract
The present study was conducted to evaluate the effects of pregnant cow urine, known to contain estrogen, on follicular dynamics in female Wistar albino rats. The rats were reared from twenty day of age for 60 days in four groups consisting of six rats in each group. First trimester pregnant cow urine and last trimester pregnant cow urine was administered at a dose of 15 ml/ kg b.w to Group III and Group IV respectively. Group II was administered with 15 ml / kg b.w of non pregnant cow urine where as Group I was administered with distilled water which served as control. Significant advancement in the mean age of attainment of puberty was observed in the Group IV compared to other groups and the average number of surface follicles in left ovary was significantly high in Group IV compared to Group I and Group II. These results were indicative of positive effect of estrogenic compounds present in pregnant cows urine on folliculogenesis in rats.

Key words: Pregnant cow urine, estrogen, pubertal age, folliculogenesis.