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Original Article

J App Pharm Sci. 2015; 5(10): 080-084


Phenylalanine enhances Quercetin content in In vitro cultures of Abutilon indicum L.

Ramgopal Rao Sajjalaguddam, Anitha Paladugu.


Abstract

Flavonoids have aroused considerable interest because of their potential beneficial effect on human health. Quercetin is one of the most abundant natural flavonoids present in medicinal plants. In the present study, a comparative phytochemical analysis of bioactive compounds from leaf and leaf derived callus of Abutilon indicum L. was carried out. Thin layer Chromatography (TLC) and High Performance Liquid chromatography (HPLC) analysis of methanolic extract of Abutilon leaf revealed the presence of Quercetin flavonoid. To test the effect of elicitor in enhancing flavonoid content, in vitro studies were conducted by supplementing phenylalanine to actively growing callus cultures of Abutilon. More than threefold increase in Quercetin content was obtained in elicitor induced callus compared to control.

Key words: Abutilon indicum Callus; Flavonoids; Quercetin; HPLC; Elicitor; Phenylalanine






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