The study was carried out to assess effective antibacterial concentration of 70% alcoholic hot extract of Piper nigrum fruit against pathogenic strains of E.coli (MTCC 96), Staphylococcus aureus (MTCC 96), Streptococcus pyogenes (MTCC 442), Proteus mirabilis (MTCC 1429) by Pelczar method. The present study revealed that E.coli and Proteus mirabilis are highly susceptible to the 70% alcoholic hot extract of Piper nigrum fruit as compared to other bacteria viz. Staphylococcus aureus, Streptococcus pyogenes.
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