Standardization of herbal medicines and plant ingredients with reference to their active marker is the need of the time. We tried to standardize Asparagus racemosus using high performance thin layer chromatography method (HPTLC) for its active compound, shatavarin IV. A simple, sensitive HPTLC method was developed for the determination of shatavarin IV in Asparagus racemosus extract. The Rf value of shatavarin IV were found to be 0.44. The amount of shatavarin IV present in the test extract is 0.23/100mg. Shatavarin IV showed good linearity in the concentration range of (32.20-1030.50 µg/mL), with a correlation coefficient of 0.999. The average percentage recovery for shatavarin IV is 99.60 %. The proposed method is reproducible and statistically validated. The system was successfully used to investigate the presence of the shatavarin IV in Asparagus racemosus plant parts as well as to analyze their content in market products.
Key words: KAsparagus racemosus, Shatavarin IV, HPTLC analysis, Method validation.