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Epidemiological and virological characterization of influenza A virus subtype H1N1 at tertiary-care hospital, Ahmedabad

Nidhi Barot, Mahendra Vegad, Sumeeta Soni, Lavanya Devi V, Vibhuti Patel.


Abstract

Background: In April 2009, a new strain of influenza virus, A H1N1, started to spread in various parts of the world, and the first case was reported on May 16, 2009.[1] The associated morbidity and mortality have made it a major health burden. In this study, we have investigated samples of patients with suspected influenza-like illnesses (ILIs) received at civil hospital, Ahmedabad, from January 1, 2015 to March 15, 2015.

Objective: To study the epidemiologic and virologic profiles of patients found positive for influenza A H1N1 at a tertiary-care hospital.

Materials and Methods: Nasopharyngeal and oropharyngeal swab specimens from patients presenting with ILI were received and subjected to real time RT-PCR (rRT-PCR) for detection and characterization of swine influenza and other seasonal influenza.

Result: Of the 6,197 specimens tested, 3,242 and 866 yielded swine H1N1 and seasonal influenza virus, respectively. Most specimens were received from children aged

Key words: H1N1, real time reverse transcriptase polymerase chain reaction (rRT-PCR) assay, tertiary-care hospital






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