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Rumen fermentation patterns in buffalo bulls fed total mixed ration supplemented with exogenous fibrolytic enzyme and/or live yeast culture

Ravikanth Reddy Poonooru, Srinivasa Kumar Dhulipalla, Raghava Rao Eleneni, Ananda Rao Kancharana.


The objective of this study was to evaluate the effect of exogenous fibrolytic enzyme (EFE) and/or live yeast culture supplementation in total mixed ration (TMR) on rumen fermentation patterns in buffalo. For this, four adult buffalo bulls weighing 377.05±43.36 kg were randomly allotted to four dietary treatments viz., TMR containing R:C ratio of 70:30 (T1), T1 supplemented with EFE at 15 g/animal/day (T2), T1 supplemented with live yeast culture at 10 g/animal/day (T3), and T1 supplemented with EFEs at 15 g/animal/day and live yeast culture at 10 g/animal/day (T4). Rumen liquor from the fistulated animals was collected at 0, 1, 2, 4, 6 and 8 h post-feeding, and was analyzed. This study revealed that rumen pH values were highest at 0 h, and were declined to minimum by 4 h post-feeding, while total volatile fatty acids (TVFA), ammonia nitrogen (NH3-N), and nitrogen (N) fractions reached to peak at 4 h post-feeding, and later followed a decreasing trend in all the treatments. Supplementation of EFE in TMR (T2¬) had no effect (P>0.05) on rumen pH and food and protozoal N concentration, while it influenced to increase (P

Key words: Buffalo bulls, exogenous fibrolytic enzymes, Live yeast culture, Rumen fermentation pattern, Total mixed ration

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