Abstarct
Coagulase positive staphylococci (CPS) and coagulase negative staphylococci (CNS) are the most common cause of bovine intramammary infection and the objective of this paper is to investigate the role of staphylococci in bovine mastitis and to study some of its virulence genes. In this study out of 318 mastitic animals examined, 22.6 % are infected with staphylococcus aureus and 12.5% of mastitic cases were infected with CNS. Concerning subclinical mastitis cases, CPS was isolated from48.2% of cases while CNS was isolated from lower percentage 8.9%. High rates of resistance to antimicrobial agents was observed in CPS and CNS. PCR was performed to detect genes Sea, sec, see and tst on 19 staphylococci isolated. As shown in fig (1) 14 samples were positive for SEC toxin.. All samples were negative for sea, see and tst toxin. This study concluded the high significant role of staphylococci in bovine mastitis in Behera Governorate and its high resistance to antimicrobial. No specific virulence factor or combination of factors has been strongly associated with the severity of mastitis
Key words: Key words: staphylococci, coagulase positive, coagulase negative, PCR, SEC toxin, Sea,see and tst toxins, antimicrobial resistance
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