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Egypt. J. Exp. Biol. (Zoo.). 2015; 11(1): 37-42


TEMPORAL AND SPATIAL EXPRESSION PATTERNS OF SAPR IN THE FRUIT FLY DROSOPHILA MELANOGASTER (DIPTERA: DROSOPHILIDAE)

Samar E. El-Kholy, Wesam S. Meshrif.


Abstract

Temporal and spatial expression patterns of saposin-related protein receptor (SapR) were determined in Drosophila melanogaster using GAL4/UAS system. The first step was to generate Drosophila line expressing SapR conjugated with GAL4. Then, the resulting line was crossed to UAS-GFP line. The GFP expression pattern in the first generation was monitored and analyzed in details. The results revealed that SapR was expressed in salivary glands, trachea, Malpighian tubule and CNS of larvae, compound eyes, oenocytes and the base of tactile sensillae of adults. GFP was also detected in intestine of both larval and adult stages.

Key words: Drosophila, GAL4/UAS system, temporal and spatial expression patterns, immunohistochemistry, SapR






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