This study aimed to assess the oxidative damage in the brain of mice exposed to chronic gasoline vapor inhalation and the protective roles of green tea extract, and powdered curcumin. Gasoline vapor inhalation caused significant signs of oxidative stress in brain tissue of mice. A significant decrease in the activity of the antioxidant enzymes SOD, CAT, GPx, and GST, and in the nonenzyme antioxidants, total thiol (T-SH), protein thiol (P-SH) and non protein thiol (Np-SH) levels was recorded. The levels of markers for protein oxidation (protein carbonyl group PCO), advanced oxidation protein product (AOPP) and lipid oxidation (lipid peroxidation, TBARS) were significantly increased. Green tea or curcumin has variable protective effects on the oxidative stress of gasoline vapor inhalation. A significant decrease in the activity of the antioxidant enzymes except for CAT was recorded. The levels of T-SH and Np-SH thiols were replenished but P-SH level was still below the control value. Protein oxidation returned to the normal levels. Lipid peroxidation occurred but at lower levels than in gasoline treated mice.
Key words: Green tea, curcumin, gasoline, thiol, mice.