Protein electrophoretic patterns and free pool amino acids of Biomphalaria alexandrina snails treated with sub-lethal concentration (10 ppm) of Roundup herbicide (48% Glyphosate) and/or Schistosoma mansoni were investigated. Comparative analysis of soluble protein of ovotestis and hemolymph was carried out using SDS-PAGE. Protein analysis showed several qualitative and quantitative differences in protein profiles. In general, the results clearly revealed that the total protein intensity was remarkably decreased in treated–infected and non-infected snails through 4 weeks post-exposure (PE). A remarkable 28 kDa band was detected in ovotestis separated protein profiles of treated and treated-infected snails during the whole exposure period of experiment. In the hemolymph, 170 kDa and 167kDa protein bands appeared only in treated non-infected snails at the 1st, 2nd, and 3rd week PE. While in treated-infected snails 168 kDa protein band appeared at the 1st, 2nd, and 3rd week PE. Analysis of the free pool amino acids by High Performance Thin Layer Chromatography (HPTLC) of ovotestis and hemolymph of B. alexandrina treated and/or treated-infected was performed at the 4th week PE. The qualitative analysis revealed the presence of 17 amino acids (Aspartic, Glutamic, Serine, Glycine, Histidine, Arginin, Theronine, Alanine, Proline, Tyrosine, Valine, Methionine, Cystine, Isoleucine, Leucine, Phenylalanine, and Lysine). The quantitative analysis of free-pool amino acids in ovotestis of B. alexandrina snails treated with Roundup showed a significant decrease in Aspartic, Proline and Cystine compared with control. Herbicide treatment and S. mansoni infection showed a decrease in Aspartic, Glutamic, Arginin, Theronine, Cystine, Phenylalanine, Valine, Proline and Iso-leucine of ovotestis in comparison with control groups. Free-pool amino acids in hemolymph showed marked decrease in Glycine, Alanine, Proline, Tyrosine, Valine, Methionine, Phenyalanine and Lysine of treated group. Treated-infected group showed a remarkable reduction in all tested amino acids except Iso-leucine and Cystine, which was not affected when compared with control group.
Biomphalaria alexandrina snails- herbicide-Roundup- Schistosoma mansoni -Free pool amino acids- SDS-PAGE - HPTLC