Isolation and characterization of Haloferax mucsum from Red sea saltern as an halobacterial Archea capable of growing on Naphtaline as an aromatic compounds.
Hanan Abd-el Fatah Temerk.
Abstract
An extremely halophilic (archeobacterium) designated strain as (H 2017) was isolated from saltern bond collected from Red sea coast (PH rang 7- 7.5) by using a Classic halophilic enrichment media (CHM).The colonies for this strain have irregular big pinky mucous sticky colonies on solid media, whereas the cells of this strain are gram negative, pleomorphic with irregular shapes ,non- motile able to grow at temperature ranging between 30-50°C (optimum 37°C), the optimum PH for growth is (PH 7.5), the optimum concentration of sodium chloride is (3.5 M NaCl ) and the cells are lysis in distilled water or in hypotonic solution, Based on high sequence similarity of their 16S rRNA genes to those of type strains of the genus Haloferax forming 99% related to Haloferax mucosum, the halobacterial isolates (H2017) have ability to biodegrade aromatic hydrocarbon and grow in the presence of naphthalene as sole source of carbon (100mg/L) ,Naphthalene is a polycyclic aromatic compound composed of a fused pair of benzene rings with formula (C10H8 ) are widely distributed and relocated into natural environment due to human activity causing pollution to the ecosystem.
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