Glyphosate herbicide was evaluated for acute toxicity. A commercial preparation containing 480 gm/l Glyphosate was applied on Clarias gariepinus in a three acute doses (600, 800, and 1000 mg/l). LC50 value was determined as 720 mg/l. Three sub lethal doses were examined for 15 days (25, 50, and 100 mg/l). Biochemical Cytogenetic and histopathological analyses were carried out to clarify the potential hazards of environmental agrochemi - cal pollution stress of Glyphosate. Increase in lipid per oxidation product (thiobarbituric acid reactive substance), reduced glutathion, and the decrease in total protein in liver appeared in high correlation with the increase in glyphosate concentration , variation between groups was significantly indicated by ANOVA analysis (p < 0.05). Chromosomal aberrations (centromeric attenuation, centric fusion, breaks and gabs) could be significantly correlated to the increase in toxicity concentration of glyphosate (p < 0.05). Histopathological pictures included gills filament cell proliferation, adhesion, cell hyperplasia and other degenerative changes in skin and internal organs. Thus, Glyphosate is supposed to exert some cytotoxicity, lipid peroxidation, enzyme alteration and histopathological lesions.
Key words: Biochemical, Clarias gariepinus, Cytotoxic, Glyphosate, Histopathology, Pollution
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