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Egypt. J. Exp. Biol. (Zoo.). 2007; 3(0): 325-331


IMMUNOCYTOCHEMICAL ASSESSMENT OF PRIMARY GRANULE DEGRANULATION IN NEUTROPHIL OF PATIENTS WITH CHRONIC RENAL DISEASE

Ezz M. El Gamal.




Abstract

Neutrophils play a major role in the development and progression of autoimmune diseases such as systemic lupus erythematosus (SLE) via the interaction of released Myeloperoxidase (MPO) from primary granules with reactive oxygen species. The activation of neutrophil leads to the exocytosis process (degranulation) to release its contents to produce toxic actions. This process can be estimated by measuring the degranulation product MPO and the selective upregulation of granule membrane molecules such as CD63. In this study, immunocytochemical localization of neutrophil degranulation product MPO and surface marker (CD63) of primary granules were investigated in patients with chronic renal disease. Neutrophil CD63 and MPO were investigated in 20 patients with systemic lupus erythematosus, 20 chronic renal failure patients (CRF), 20 kidney transplanted recipients in case of normal kidney function (NKF) and during episodes of acute rejection (A-Rej) and during chronic rejection (C-Rej) and 20 normal healthy controls free of liver and kidney disease using immunofluorescence technique. Patients with SLE and CRF exhibited an increase in degranulation product MPO and marker CD63 as reflected by strong immunoreactivity and significant increase in the number of positive neutrophils compared to the control. In contrast, in renal transplanted recipients there was a significant decrease in CD63 and MPO positive neutrophils in case of normal kidney function; however there was a significant increase in the number of positive neutrophils during episodes of acute and chronic rejection. There was a significant correlation between CD63 and MPO of primary granules within neutrophil in SLE and CRF and during episodes of acute rejection which is considered one of the crucial reasons of renal dysfunction. The assessment of neutrophil activity by demonstrating the changes in the degranulation products (MPO) and surface marker (CD63) of primary granules can be used as useful marker to monitor the renal disease.

Key words: Neutrophil – Degranulation – CD63 – SLE - Renal transplantation – Acute rejection.






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