Ultrastructure study, using semi-thin sections and electron micrographs, was carried out to demonstrate the ovicidal effect of chitin synthesis inhibitor (Lufenuron) and acetonic extract of rice bran, Oryza sativa on embryogenesis of the house fly Musca domestica. For this purpose the embryonic development of the normal house fly eggs was studied using ultrastructure technique during cleavage, blastoderm and gastrulation. Cleavage started 15 minutes postoviposition (POP) and continue to divide until cellular blastoderm is formed 2 hours later. After 3 hours, the germ band is formed, differentiated into ectoderm and mesoderm. At 4 hours, while the embryonic envelopes are formed, gastrulation of the germ band takes place. Ultrastructure study revealed that application of both compounds to newly Musca domestica laid eggs (0-15 minutes) blocked the embryonic development at cleavage stage and before the blastoderm and pole cells formation. Extensive cellular and tissue damage of the developing embryo was observed.
Musca domestica, chitin synthesis inhibitor (Lufenuron), plant extract, rice bran (Oryza sativa), embryogenesis, ultrastructure