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Egypt. J. Exp. Biol. (Zoo.). 2012; 8(2): 349-354


ASSESSMENT OF MULTIDRUG RESISTANCE GENES BY MULTIPLEX PCR IN BREAST CANCER PATIENTS

Mohammed N. Karim Nidhal A. Mohammed.

Abstract
Breast cancer (BC) is the most common cause of cancer death among women worldwide. The current rates of increase remain constant; a woman born today has a 1 in 10 chance of developing breast cancer. The response of breast cancer patients to the course of chemotherapy, hormonal therapy and radiotherapy is depending on the presence or absence of one or many multidrug resistant genes in their genome (MDR). This prospective study was carried out to investigate the possible association of multidrug resistant genes with resistance to chemotherapy in BC patients. A total of 50 female patients with BC were studied, their ages range from 21-65 year with a mean age of 48 10.36 years. Two control groups were included in this study for comparison. The first group included 10 patients with benign breast lesions 8 fibroadenoma and 2 fibrocystic disease and 6 apparently healthy individuals. Breast tissue samples were collected from patients and controls, mRNA was extracted, and then reverse transcriptase polymerase chain reaction was performed then multiplex polymerase chain reaction (MPCR) using a specific sequence primers for multidrug resistant genes (MDR) to observe the possible association of certain MDR genes including: human ApoD, CRYAB, CYR61, G1P2, G1P3, IFITM1, IFITM3, IL1-beta, PDE3A, PEPP2, PLAB and UBB genes. There were significant differences between the following MDR genes (IFITM1 & PEPP2), as well as high significant difference of CRYAP, UBB and IFITM3 genes of BC patients as compared with healthy and control patients. In addition, there was highly significant correlation coefficient between the IFITM1 & PEPP2 genes and the stage and grade of BC, as well as a highly significant correlation coefficient between IFITM1 gene and PEPP2 gene, PDE3A gene, and CYR61 gene, CRYAP gene with UBB gene, CRYAP gene and IFITM3 gene, UBB gene and IFITM3. Also there were correlation coefficients between PEPP2 gene and PDE3A gene, and between PEPP2 gene and CYR61gene.

Key words: Breast Cancer, Multidrug resistant genes, Multiplex PCR



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